Insertion junction: LMJ.RY0402.205378_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205378
Chromosome:chromosome 4
Location:496002
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGTGAGTGTGGAGCGCTGGCCCGTGTTT
Internal bar code:CGCCGCGACTCGGTACAAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:705
LEAP-Seq percent confirming:99.793
LEAP-Seq n confirming:4821
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCTGCGTACATGGAGTC
Suggested primer 2:GCGTAATGTCGTGTGTTTGG