| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205426 |
| Chromosome: | chromosome 16 |
| Location: | 5721530 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679051 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACGCCCTGTCCTGCCCTCACAACCCGC |
| Internal bar code: | GGACCCTCTTGACTGGGCGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.8447 |
| LEAP-Seq n confirming: | 643 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCAGTCAAGGACGCATA |
| Suggested primer 2: | GGACGACTCGCCAGATAAAA |