| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205473 |
| Chromosome: | chromosome 12 |
| Location: | 3295138 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497950 | (1 of 1) PTHR10706 - F-BOX FAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTCCTGCAGCGCCAGGCCCTGCTGCG |
| Internal bar code: | GTTGCATCGCGGGCTTGTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 97.2149 |
| LEAP-Seq n confirming: | 733 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTACTCGAACACCAGCTC |
| Suggested primer 2: | CAGCAAATAGGAGGGCTCAG |