| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205480 |
| Chromosome: | chromosome 1 |
| Location: | 6548986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGTGGCCCCGCTGCTGAATACTCAT |
| Internal bar code: | CAAACCCGGGATTGATATGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 73.3561 |
| LEAP-Seq n confirming: | 8378 |
| LEAP-Seq n nonconfirming: | 3043 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGCACCAACGACAACAC |
| Suggested primer 2: | GTCCTGTGCTTTGTAGGGGA |