Insertion junction: LMJ.RY0402.205481_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.205481
Chromosome:chromosome 7
Location:830126
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g318500 CYA8 Putative very-long-chain fatty acid condensing enzyme; (1 of 5) 2.3.1.199 - Very-long-chain 3-oxoacyl-CoA synthase / Very-long-chain beta-ketoacyl-CoA synthase 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGTGGCTGTGGCCTGCTGTGATTGGGC
Internal bar code:TGCCATTCCTGGATTTACAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:467
LEAP-Seq percent confirming:99.651
LEAP-Seq n confirming:1999
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGGCTAGTCGAGTTGGG
Suggested primer 2:CTGCAAACATTTTGTGTGGG