Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.205481 |
Chromosome: | chromosome 7 |
Location: | 830126 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g318500 | CYA8 | Putative very-long-chain fatty acid condensing enzyme; (1 of 5) 2.3.1.199 - Very-long-chain 3-oxoacyl-CoA synthase / Very-long-chain beta-ketoacyl-CoA synthase | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTGGCTGTGGCCTGCTGTGATTGGGC |
Internal bar code: | TGCCATTCCTGGATTTACAACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 467 |
LEAP-Seq percent confirming: | 99.651 |
LEAP-Seq n confirming: | 1999 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAGGCTAGTCGAGTTGGG |
Suggested primer 2: | CTGCAAACATTTTGTGTGGG |