| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205493 |
| Chromosome: | chromosome 3 |
| Location: | 7544176 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205150 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTGTGTCATGCGTATGCGTCCCACGTG |
| Internal bar code: | AACCAGGAGGCGAAAATGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 855 |
| LEAP-Seq percent confirming: | 79.6385 |
| LEAP-Seq n confirming: | 3833 |
| LEAP-Seq n nonconfirming: | 980 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGCTGGAATTTGGCAAT |
| Suggested primer 2: | TCCTCACACACCTGCTGTTC |