Insertion junction: LMJ.RY0402.205509_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.205509
Chromosome:chromosome 13
Location:3734165
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTCGCGTGCGGCGGCGCGGGGCTTGAC
Internal bar code:GGACAACACACACAAGTAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:832
LEAP-Seq percent confirming:99.2414
LEAP-Seq n confirming:1439
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCGCTACAGTGACAA
Suggested primer 2:CAACAAGAACTACCGTCGCA