| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205513 |
| Chromosome: | chromosome 11 |
| Location: | 1891053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468100 | PDE30 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTTGGGTTTCTTGGGCTGCTGTCACT |
| Internal bar code: | GCCCACATGAAGCTCACGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 99.6072 |
| LEAP-Seq n confirming: | 1268 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCTTACTCGTTGCTATC |
| Suggested primer 2: | ATGCACATGCCATAGACCAA |