| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205555 |
| Chromosome: | chromosome 17 |
| Location: | 2696303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g717900 | PHC1 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGAGAGGGGTTGGGTTTGCCCTGGTG |
| Internal bar code: | CGGGGCTCGTTCCTTTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.3283 |
| LEAP-Seq n confirming: | 3697 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCACCCTGAACGACTTC |
| Suggested primer 2: | CGGAGTCTTTGTTGCAGTCA |