| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205564 |
| Chromosome: | chromosome 4 |
| Location: | 2903949 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224550 | GT90F37,GT90-37 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 37 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAAAGATGTTGACGCGCGACCTGCTGT |
| Internal bar code: | TCTTGCCTTCTCCCCAGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 90.9117 |
| LEAP-Seq n confirming: | 3141 |
| LEAP-Seq n nonconfirming: | 314 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCTGCTTCATGTCGTC |
| Suggested primer 2: | ACACCCCCATCCCTCTTATC |