Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.205579 |
Chromosome: | chromosome_16 |
Location: | 3545371 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g678808 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGTCGTGTTGGTGCCTTGCGGGTGAAGCG |
Internal bar code: | GACAATTATAATGCTACAAGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 370 |
LEAP-Seq percent confirming: | 99.3483 |
LEAP-Seq n confirming: | 2439 |
LEAP-Seq n nonconfirming: | 16 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCAATTTCGCATAGACAGA |
Suggested primer 2: | GACGTTGTGCATCTTTGTGG |