Insertion junction: LMJ.RY0402.205582_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205582
Chromosome:chromosome 7
Location:1382794
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g322600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTCCAGGTACTGCGTGGCCCTGGCGGTG
Internal bar code:CCAAGCATGGATCGACGTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:432
LEAP-Seq percent confirming:79.0902
LEAP-Seq n confirming:1982
LEAP-Seq n nonconfirming:524
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGCAAGACACGAATAGTT
Suggested primer 2:AGATCTACAACGCCACCACC