Insertion junction: LMJ.RY0402.205583_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.205583
Chromosome:chromosome 2
Location:5160048
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g105400 CDC14 (1 of 1) K06639 - cell division cycle 14 (CDC14); Cell Division Cycle protein 14 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAATGTAATGTCACATACCCACACATGTC
Internal bar code:TAATCGCAATGGAAACGGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1017
LEAP-Seq percent confirming:99.7827
LEAP-Seq n confirming:5052
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTACTAGGCAACCCTGAG
Suggested primer 2:TGCAGCTTTTTACATGGCAG