| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.205585 |
| Chromosome: | chromosome 1 |
| Location: | 1711036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009150 | (1 of 1) PF14329 - Domain of unknown function (DUF4386) (DUF4386) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCACAGAGTTGCTGCAGTTGGGTTTCG |
| Internal bar code: | CCGCCCGGGCTAAGCAGATCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 98.2249 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAGTTGCAGGAGGATAC |
| Suggested primer 2: | CACCTTGGAAGGCGTAGAAG |