| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205588 |
| Chromosome: | chromosome 11 |
| Location: | 2493033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475700 | (1 of 140) IPR002048 - EF-hand domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAGCCGATGCCACCGCTGCAGGGAATG |
| Internal bar code: | GAAGACGGGGGTGGTTAGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 99.6534 |
| LEAP-Seq n confirming: | 6900 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCAGCCTAAAAGTGAC |
| Suggested primer 2: | CAATCTTTTGCAGTGAGCGA |