Insertion junction: LMJ.RY0402.205609_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205609
Chromosome:chromosome 3
Location:8707302
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g204465 GT90F36,GT90-36 GT90 family protein 36; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTACATTACGGTACCCCTGAGCACGGCCG
Internal bar code:GTGAGGGGGGTCGACATACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:846
LEAP-Seq percent confirming:98.1272
LEAP-Seq n confirming:3301
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CATTTTGGCCGTTTCCTAGA
Suggested primer 2:ACACGAGATTGTGCTTGTGC