Insertion junction: LMJ.RY0402.205655_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.205655
Chromosome:chromosome_9
Location:3667949
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g390615 FAP12 Triacylglycerol lipase and Flagellar Associated Protein antisense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCACACTACTCGCGCACACCATCTTGCTTC
Internal bar code:AGTGCTCCCATCAGCAGCAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:931
LEAP-Seq percent confirming:98.4099
LEAP-Seq n confirming:557
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGAAAGGGAGCAGACGTG
Suggested primer 2:GTTGCAAGAATGCGAAGACA