| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205659 |
| Chromosome: | chromosome 8 |
| Location: | 1519800 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364800 | (1 of 1) 6.3.5.3 - Phosphoribosylformylglycinamidine synthase / Phosphoribosylformylglycinamidine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTCTGGCAAAAAGCGTTGATGCGGCG |
| Internal bar code: | TGGTTATAACCCGGTAGCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.6146 |
| LEAP-Seq n confirming: | 2585 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGTTCTACGTGCCTGC |
| Suggested primer 2: | TGAGTTGCGCATTTTGTAGC |