| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205702 |
| Chromosome: | chromosome 3 |
| Location: | 2844517 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162500 | (1 of 4) IPR011016//IPR013083 - Zinc finger, RING-CH-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGTTGTGTGTTGGATTCGGGCATTGT |
| Internal bar code: | TCGTTTATTGTCAAGCGTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 995 |
| LEAP-Seq percent confirming: | 99.1304 |
| LEAP-Seq n confirming: | 1938 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTTACTGTGCGGTTTT |
| Suggested primer 2: | CCTGATCAACACCCAGGACT |