| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205704 |
| Chromosome: | chromosome 3 |
| Location: | 3700199 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169350 | (1 of 3) IPR007860 - DNA mismatch repair protein MutS, connector domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGGCTTCCGCGTCGCTTGTTATCATG |
| Internal bar code: | ACACTCGGCAAGGCAGGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 936 |
| LEAP-Seq percent confirming: | 99.8285 |
| LEAP-Seq n confirming: | 582 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGTTGTGCGCCAGTAAA |
| Suggested primer 2: | TAAGCTCGCACCAACATCAG |