| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205708 |
| Chromosome: | chromosome_10 |
| Location: | 5827744 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g461575 | antisense | 3'UTR|CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAGCCCGCAAGCACGCACCGTGGTTGCTC |
| Internal bar code: | CGGGGAAAGCCAGTTCAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 99.5327 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCACCTGGTTGATGAAA |
| Suggested primer 2: | CCCAATCTTGATTGCAGGTT |