| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.205710 |
| Chromosome: | chromosome 14 |
| Location: | 1815163 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620150 | (1 of 1) PTHR10120//PTHR10120:SF21 - CAAX PRENYL PROTEASE 1 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACACGAGTTCGGGCCGCTAAACCGGCAG |
| Internal bar code: | ACGATCCGATTAATTCTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 99.8559 |
| LEAP-Seq n confirming: | 693 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGCTGATGTTCTCAAGC |
| Suggested primer 2: | TTTCAGGTGTGTGCGAAGAG |