| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205727 |
| Chromosome: | chromosome 3 |
| Location: | 8178494 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200351 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCTCCCCTCCCTCCCTCCATCATCCC |
| Internal bar code: | GCCAGCCATGGATCGCTTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 84.0909 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCAAGAGAAGAACACCG |
| Suggested primer 2: | ACACACCACGGTGTTGAAGA |