Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.205729 |
Chromosome: | chromosome_8 |
Location: | 3432937 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g374700 | FAP244 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCGATAAAAGTGATCACGACGACTGAAGC |
Internal bar code: | ACCCCCGCAGGACAAACGATAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 612 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 315 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGGACTACAAACTGCCG |
Suggested primer 2: | TGGCTGAGGCTGTAACTGTG |