| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.205739 |
| Chromosome: | chromosome 1 |
| Location: | 6842659 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049350 | (1 of 1) PTHR31412//PTHR31412:SF5 - FAMILY NOT NAMED // ZINC METALLOPROTEASE EGY2, CHLOROPLASTIC-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCGGGTTAACGCCTTGAGTTTCCCTT |
| Internal bar code: | CTCGGCTCGAGCCGTGTATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1062 |
| LEAP-Seq percent confirming: | 99.6055 |
| LEAP-Seq n confirming: | 1515 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGGGCTACTTGAGGGCT |
| Suggested primer 2: | ACCCGTGTTATCAAGCCAAG |