| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205746 |
| Chromosome: | chromosome 17 |
| Location: | 3444923 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724650 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGATGGGTTGGGGTCGGCAGGCCGCCAC |
| Internal bar code: | GCCCATATGTGGAGTGGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 55.7572 |
| LEAP-Seq n confirming: | 10324 |
| LEAP-Seq n nonconfirming: | 8192 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGAGAAAGCCGTAACAG |
| Suggested primer 2: | AGCGGTGTATGGGATCTTTG |