| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205751 |
| Chromosome: | chromosome_3 |
| Location: | 4790201 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g178500 | RIB43,RIB43A | Flagellar protofilament ribbon protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTCTCCCAAACCAGCCCAGCGCCCTGTCC |
| Internal bar code: | AGTCACCGCCCTCCGGGCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 99.5273 |
| LEAP-Seq n confirming: | 3158 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGCTTCTACCTTCTGCG |
| Suggested primer 2: | GTGGTGCGGAGAAATGAGAT |