| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205763 |
| Chromosome: | chromosome 10 |
| Location: | 2196101 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTCTGCGGCACGCTCGAACATATGAGT |
| Internal bar code: | CTTCTCTTCACACGAGTTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.7585 |
| LEAP-Seq n confirming: | 413 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGATTGTTGGACCCGTTC |
| Suggested primer 2: | CACGGTTGACATGACTGGAC |