| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205780 |
| Chromosome: | chromosome_17 |
| Location: | 3962520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728700 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCTCACGGAGGGCGATGGGCGCGGCCTGA |
| Internal bar code: | ATCTAGCGACTGGGACACCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 391 |
| LEAP-Seq percent confirming: | 99.8796 |
| LEAP-Seq n confirming: | 2488 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGCGACTTCCTTGTCA |
| Suggested primer 2: | GATTTTGATCTGGGTCGGAA |