| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205783 |
| Chromosome: | chromosome 5 |
| Location: | 1328380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244750 | (1 of 1) K13205 - A1 cistron-splicing factor AAR2 (AAR2, C20orf4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATGTAGATGGAAGATACGAGCACTGGC |
| Internal bar code: | CTTCGGTGACTAATCGCCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 98.5093 |
| LEAP-Seq n confirming: | 1586 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGATCACCCTCAAACCG |
| Suggested primer 2: | TTTCAGAGCGGGTAGCTGAT |