Insertion junction: LMJ.RY0402.205784_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.205784
Chromosome:chromosome 4
Location:2361090
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g220900 GT90-4,GT90F4 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAATAACAGGGCTCCTGCTGGAAGGTCTC
Internal bar code:ATCACACGCTCCAGACAGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:960
LEAP-Seq percent confirming:99.5809
LEAP-Seq n confirming:5703
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTCGTTTCATGTGGTGC
Suggested primer 2:CATAATGCTCAGCGCAGGTA