| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205786 |
| Chromosome: | chromosome 7 |
| Location: | 2425271 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328850 | CNK8 | NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCCGAGCCGCAGGTGTGGGCCTGGCT |
| Internal bar code: | GTGCACCCGCTTACGCGCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 99.6207 |
| LEAP-Seq n confirming: | 788 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGAGCTAAAGCGTCAGG |
| Suggested primer 2: | TACGGCTGCTCCTCGTAGAT |