| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205794 |
| Chromosome: | chromosome 1 |
| Location: | 3156251 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019700 | PAP7 | Class-II RNA nucleotidyl transferase 7; (1 of 12) 2.7.7.19 - Polynucleotide adenylyltransferase / RNA adenylating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCTGGCGTGGGGGCGCTAGGCACGCAGC |
| Internal bar code: | CCGGGGGGCGTACACCCAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.8819 |
| LEAP-Seq n confirming: | 2538 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGCCATAGCACAATAGAA |
| Suggested primer 2: | GGCGCGTACCAAGTAAAAGA |