| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205831 |
| Chromosome: | chromosome 16 |
| Location: | 497215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGACGTTGCAGAACGCCTGGGTGGCGC |
| Internal bar code: | CAGGACATTCGCTTAAATACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 93.9262 |
| LEAP-Seq n confirming: | 433 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGGTGTGTCTGCGAGTG |
| Suggested primer 2: | CTGGCAGGGCAAGATACAAT |