| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205835 |
| Chromosome: | chromosome 17 |
| Location: | 3439972 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724600 | PAO2,TIC55-2 | Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGAGCAAAGAGGCGGGTATTGACTGT |
| Internal bar code: | GAGTAGTCGCTTCACACCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.5246 |
| LEAP-Seq n confirming: | 2512 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCACCAGCACACCAAAAA |
| Suggested primer 2: | AAGCGAGAGGCTGTGAACAT |