| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.205898 |
| Chromosome: | chromosome 12 |
| Location: | 2984749 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501150 | MLT2 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype; Multi eye protein 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTTAATCCTACACTACTTGCTTGCTGC |
| Internal bar code: | CTAGAAAGACGGTTGGTAGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 99.6721 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCTTTTGACGGTCTCA |
| Suggested primer 2: | AGCGCATGTTTGAGGACTTT |