| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205916 |
| Chromosome: | chromosome 7 |
| Location: | 3858985 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGGAGCTCTGCTAACTATACCCTAAC |
| Internal bar code: | CCCGGGTTCGGGCACGCCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.5599 |
| LEAP-Seq n confirming: | 2941 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATCTCCGCGTCACTAC |
| Suggested primer 2: | CGGTTGGTTGCTATGTTCCT |