| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205919 |
| Chromosome: | chromosome 16 |
| Location: | 850003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647800 | (1 of 1) IPR000104//IPR001932//IPR015655 - Antifreeze protein, type I // PPM-type phosphatase domain // Protein phosphatase 2C | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGTCCGCCGCGTGACCCGCGCCTCAC |
| Internal bar code: | CTTCGGGTAATGATGGAGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 378 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCTCCAACACAATCACC |
| Suggested primer 2: | TCTAGAGGCGGTAGCGTGAT |