| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205945 |
| Chromosome: | chromosome_17 |
| Location: | 1038523 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g703600 | OFD1 | Basal body protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGGGGCACGCTTGGTGTGGCGAGGGAGG |
| Internal bar code: | CTCTAACCTTCGCACCATGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.8683 |
| LEAP-Seq n confirming: | 4551 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATTGCAAGCTAGTGGTT |
| Suggested primer 2: | TAATGACGATACGCTCGCAG |