| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205965 |
| Chromosome: | chromosome 6 |
| Location: | 1352842 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258700 | PYC1 | (1 of 1) 6.4.1.1 - Pyruvate carboxylase / Pyruvic carboxylase; Pyruvate carboxylase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACACCGGCGCGCAGATGGCCGTCTCCAT |
| Internal bar code: | TAAACCAAAACCGGCATTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.7244 |
| LEAP-Seq n confirming: | 1809 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGACACCTCAGTCCTT |
| Suggested primer 2: | AGTTGCTCTTGCTCACCGAT |