| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.205975 |
| Chromosome: | chromosome 2 |
| Location: | 6273540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACGGGGGAGGGCCGCGCATGGGGTAGG |
| Internal bar code: | CACTACATGCGAAAGCAGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 733 |
| LEAP-Seq percent confirming: | 99.7605 |
| LEAP-Seq n confirming: | 5415 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCATGTCGTTCTTTCA |
| Suggested primer 2: | CCTGCAGTAGGAGCAAAAGG |