| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205990 |
| Chromosome: | chromosome 8 |
| Location: | 3222029 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373550 | (1 of 1) K01515 - ADP-ribose pyrophosphatase (nudF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTGGGAATGGTGGAAACAACGCTCCCC |
| Internal bar code: | GGGATCCGGCGGGTAAGTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.7507 |
| LEAP-Seq n confirming: | 2401 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAACCCTGAAACAGGAAC |
| Suggested primer 2: | GCGAGTACGTGTGCAAGTGT |