| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205999 |
| Chromosome: | scaffold 19 |
| Location: | 18356 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750197 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCACCTGTTTAAAAGTTTTGGATCGCA |
| Internal bar code: | ATTACTTCTCTCGGTCGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.2914 |
| LEAP-Seq n confirming: | 2382 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTCCTCCTCCTCTTTCT |
| Suggested primer 2: | GTACCCCATTTGTTGCTGCT |