| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206011 |
| Chromosome: | chromosome 13 |
| Location: | 3508665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGGCCGACGCGCATTGAGCACACACATG |
| Internal bar code: | TTCTACGGCATGCTTGGATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 98.6709 |
| LEAP-Seq n confirming: | 1856 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGTCAAAGTAAGCACTC |
| Suggested primer 2: | TTGGTGTTGACGGTGTTGAT |