| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206033 |
| Chromosome: | chromosome 9 |
| Location: | 1976578 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394800 | MITC16, MCP16 | Mitochondrial substrate carrier protein; (1 of 3) K05863 - solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31 (SLC25A4S, ANT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCACGCCATGCCGGCCCTGCATGCTGA |
| Internal bar code: | GGTTGTATCGTTGCAAAACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 95.1484 |
| LEAP-Seq n confirming: | 2020 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCACTGATTAAAAGCAC |
| Suggested primer 2: | AAACTACCACTGGCCGTGTC |