| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206054 |
| Chromosome: | chromosome 10 |
| Location: | 2571245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436850 | (1 of 7) 2.4.1.255 - Protein O-GlcNAc transferase / OGTase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACGCTGGCAAAGGGGCACTGTGCGTGT |
| Internal bar code: | TTCGATGTCATTGGGGGGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 881 |
| LEAP-Seq percent confirming: | 99.0984 |
| LEAP-Seq n confirming: | 10992 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTGATGTTAGTGCAGGT |
| Suggested primer 2: | AGCCGAGCTGCTTGTAATGT |