| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206077 |
| Chromosome: | chromosome 11 |
| Location: | 899822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467656 | (1 of 9) IPR007112//IPR009009 - Expansin/pollen allergen, DPBB domain // RlpA-like double-psi beta-barrel domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCCTTGGGCTCGTACCAGCAAGGGCAG |
| Internal bar code: | TTAGAAAGTGACTTGTGCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 99.7636 |
| LEAP-Seq n confirming: | 5064 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGCCTCTCTTCCACTTC |
| Suggested primer 2: | AAGTGGGTTCACCGTAGTCG |