Insertion junction: LMJ.RY0402.206080_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.206080
Chromosome:chromosome 3
Location:5518066
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g185950 (1 of 1) IPR006642//IPR026319 - Zinc finger, Rad18-type putative // Zinc finger C2HC domain-containing protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTTCTAGCACGTGTATCGTGTATGACA
Internal bar code:TTTATGTGACCCGCGAGGTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:64.7059
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAAAGCTGTGTGACGCCT
Suggested primer 2:GGAACAAGCAAGGACAGCTC