Insertion junction: LMJ.RY0402.206125_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.206125
Chromosome:chromosome 3
Location:293958
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g144564 MMP13 Matrix metalloproteinase; (1 of 1) IPR008752//IPR024079 - Peptidase M11, gametolysin // Metallopeptidase, catalytic domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGCTTTATTGCTGTCGGGGCTGTGGTG
Internal bar code:CCTGGGCCAGACTAATTCGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:784
LEAP-Seq percent confirming:98.3144
LEAP-Seq n confirming:2158
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAGCCCAAACTAAACCG
Suggested primer 2:CACAACCAGTCAGCCAGAGA