| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206131 |
| Chromosome: | chromosome 13 |
| Location: | 1347776 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571500 | CST2 | Chlamydomonas-specific membrane transporter of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGAACACCGCAGTGATGATGCAGCCG |
| Internal bar code: | TGGCCCGACGAGCTTGCGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 99.6465 |
| LEAP-Seq n confirming: | 2537 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCGCGGTAGTTTCATC |
| Suggested primer 2: | GCGCTGACATGATCTGTGTT |